abstract：:The de novo design of polypeptide sequences with a three-dimensional structure necessary for many biological functions is limited by the complex folding process, or 'protein folding problem'. This problem can be bypassed through constructing protein-like molecules with a 'built-in' device for intramolecular folding, t...

journal_title：Journal of biotechnology

authors： Tuchscherer G,Mutter M

更新日期：1995-07-31 00:00:00

abstract：:The filamentous ascomycete fungus Cryphonectria parasitica naturally secretes endothiapepsin, an aspartic proteinase. It is cultured on a commercial scale as a source of the milk-clotting enzyme for cheese making. Our objective was to increase enzyme production of an industrial C. parasitica strain by a new technique ...

journal_title：Journal of biotechnology

authors： Jara P,Delmas P,Razanamparany V,Olsen L,Dupin P,Bayol A,Bégueret J,Loison G

更新日期：1995-06-01 00:00:00

abstract：:Gene farming is one of the most promising areas in modern biotechnology. To assay the potential usefulness of transgenic rabbits as bioreactors, one call embryos were microinjected with a chimeric gene comprising 5' sequences from mouse whey acidic protein gene (mWAP) linked to the human growth hormone (hGH) gene. Tra...

journal_title：Journal of biotechnology

authors： Limonta JM,Castro FO,Martínez R,Puentes P,Ramos B,Aguilar A,Lleonart RL,de la Fuente J

更新日期：1995-05-15 00:00:00

abstract：:A murine B-lymphocyte hybridoma (CC9C10) was adapted for growth in a serum-free medium. Supplementation of the medium with cis-unsaturated fatty acids (10-50 microM) improved the cell yield in the order oleic/linoleic > linoleic > oleic. Initial supplementation with the fatty acids also caused a significant increase (...

journal_title：Journal of biotechnology

authors： Butler M,Huzel N

更新日期：1995-04-15 00:00:00

abstract：:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

journal_title：Journal of biotechnology

authors： Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

更新日期：1995-02-21 00:00:00

abstract：:A system has been developed for the release of heterologous proteins from Saccharomyces cerevisiae, based on the use of thermosensitive osmotic-remedial mutants, deficient in cell integrity, that lyse at the non-permissive temperature, thus releasing the bulk of intracellular proteins and leaving behind cell ghosts an...

journal_title：Journal of biotechnology

authors： Alvarez P,Sampedro M,Molina M,Nombela C

更新日期：1994-11-30 00:00:00

abstract：:We previously reported that a recombinant strain, SB412(pLGM304), was constructed from acidomycin-resistant mutants of Serratia marcescens and produced 200 mg of d-biotin per liter of a medium containing sucrose and urea (Sakurai et al., 1993a, b). In the present work, we intended to improve the d-biotin production. B...

journal_title：Journal of biotechnology

authors： Sakurai N,Imai Y,Masuda M,Komatsubara S,Tosa T

更新日期：1994-07-29 00:00:00

abstract：:A solid-phase method is described to create subclones, suitable for DNA sequencing, from lambda or cosmid libraries. The purified target DNA is sonicated and two linkers, with one oligonucleotide biotinylated in the 5'-end, are ligated to the ends of the fragments produced by sonication. After size separation, the fra...

journal_title：Journal of biotechnology

authors： Hultman T,Uhlén M

更新日期：1994-06-30 00:00:00

abstract：:Different systems are being studied and used to prepare recombinant proteins for pharmaceutical use. The blood, and still more the milk, from transgenic animals appear a very attractive source of pharmaceuticals. The cells from these animals are expected to produce well-matured proteins in potentially huge amounts. Se...

journal_title：Journal of biotechnology

authors： Houdebine LM

更新日期：1994-05-31 00:00:00

abstract：:Recombinant Escherichia coli clones encoding between 0 and 6 copies of the mammalian cytochrome b5 gene were subjected to pyrolysis mass spectrometry (PyMS). To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the amount of cytochrome b5 produced fully-interconnected feedforward artif...

journal_title：Journal of biotechnology

authors： Goodacre R,Karim A,Kaderbhai MA,Kell DB

更新日期：1994-05-15 00:00:00

abstract：:The entire human parathyroid hormone (hPTH) cDNA gene with its natural signal and pro-region is expressed in transfected mouse mammary tumor cells (C127I cells) and Chinese hamster lung cells (DON cells) under control of the murine metallothioneine-1 promoter in a vector in which replication functions are provided by ...

journal_title：Journal of biotechnology

authors： Rokkones E,Kareem BN,Olstad OK,Høgset A,Schenstrøm K,Hansson L,Gautvik KM

更新日期：1994-04-15 00:00:00

abstract：:We have produced a chimeric bispecific antibody that has dual specificity of human fibrin and urokinase-type plasminogen activator (u-PA). Complementary DNAs for variable regions of both anti-fibrin and anti-u-PA antibodies were cloned from two murine hybridomas secreting respective antibodies using polymerase chain r...

journal_title：Journal of biotechnology

authors： Tada H,Kurokawa T,Seita T,Watanabe T,Iwasa S

更新日期：1994-03-31 00:00:00

abstract：:Cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) produces cyclodextrin (CD) from starch through an intramolecular transglucosylation reaction. To obtain a better understanding of the amylolytic and cyclization mechanisms of CGTase, and furthermore to improve the production of gamma-CD, mutant CGTases were constru...

journal_title：Journal of biotechnology

authors： Sin KA,Nakamura A,Masaki H,Matsuura Y,Uozumi T

更新日期：1994-02-28 00:00:00

abstract：:Porcine muscle adenylate kinase (ADK) was overproduced in Escherichia coli using the expression plasmid with double A-T-G codon at the translational starting site and the Shine-Dalgarno (SD) sequence 10 bp apart from the first A-T-G. We used the expression vectors pKK223-3 and pMK2. pMK2 is about 10-20 times larger in...

journal_title：Journal of biotechnology

authors： Hibino T,Misawa S,Wakiyama M,Maeda S,Yazaki K,Kumagai I,Ooi T,Miura K

更新日期：1994-02-14 00:00:00

abstract：:The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product was purified and it...

journal_title：Journal of biotechnology

authors： Moguilevsky N,Guillaume JP,Varsalona F,Bulinckx M,Bollen A

更新日期：1994-01-15 00:00:00

abstract：:A reagentless carbon paste electrode chemically modified with covalently bound alcohol oxidase and horse-radish peroxidase was examined as a selective sensor in flow injection and column liquid chromatography. A combination of carbodiimide, glutaraldehyde, and polyethyleneimine was used for immobilizing the enzymes in...

journal_title：Journal of biotechnology

authors： Johansson K,Jönsson-Pettersson G,Gorton L,Marko-Varga G,Csöregi E

更新日期：1993-12-01 00:00:00

abstract：:To achieve effective bioprocess monitoring, sensing systems are required which are suitable for an on-line determination of substrates, inhibitors, nutrients or products. Such devices may utilise biochemical principles, i.e. the specific interaction of biochemical receptors with their surroundings. They can be constru...

journal_title：Journal of biotechnology

authors： Bilitewski U,Drewes W,Neermann J,Schrader J,Surkow R,Schmid RD,Bradley J

更新日期：1993-12-01 00:00:00

abstract：:Flow cytometry has been used to study the mechanisms of damage to mammalian cells by hydrodynamic forces. Cell damage resulted from the stresses created both by bubble entrainment and by bubble bursting caused by vortex formation in highly agitated culture. Damage to the antigen molecules on the cell surface together ...

journal_title：Journal of biotechnology

authors： al-Rubeai M,Emery AN,Chalder S,Goldman MH

更新日期：1993-11-01 00:00:00

abstract：:A model for transport of ammonia and ammonium ions across cell membranes is presented. The model suggests that ammonium ions compete with potassium ions for inward transport, over the cytoplasmic membrane, via potassium transport proteins like the Na+/K(+)-ATPase and the Na+K+2Cl(-)-cotransporter. It also explains the...

journal_title：Journal of biotechnology

authors： Martinelle K,Häggström L

更新日期：1993-09-01 00:00:00

abstract：:A membrane-bound xylose oxidizing PQQ-dependent dehydrogenase from Gluconobacter oxydans was purified with a simple large-scale applicable purification procedure. The activity recovery from membrane extract was 33% with 130-fold purification. Important characteristic with respect to the application of the dehydrogenas...

journal_title：Journal of biotechnology

authors： Smolander M,Buchert J,Viikari L

更新日期：1993-06-01 00:00:00

abstract：:The production of cephalosporin C (CPC) and its precursors penicillin N (PEN N), deacetoxycephalosporin C (DAOC) and deacetylcephalosporin C (DAC), with a highly productive strain of Cephalosporin acremonium, was investigated in an 80-1 airlift tower loop reactor with four static mixer modules (Type SMV, Sulzer) (ATLR...

journal_title：Journal of biotechnology

authors： Zhou W,Holzhauer-Rieger K,Bayer T,Schügerl K

更新日期：1993-04-01 00:00:00

abstract：:A hydrogen electrode based on a hydrogenase immobilized on a carbon material has been developed. The equilibrium hydrogen potential was reached in an atmosphere of H2 on the enzyme electrode according to the mechanism of direct exchange of electrons between the electrode and the enzyme active center. The electrochemic...

journal_title：Journal of biotechnology

authors： Varfolomeyev SD,Yaropolov AI,Karyakin AA

更新日期：1993-02-01 00:00:00

abstract：:Anion exchange membranes prepared by adsorption of polymers on Formed-In-Place microfiltration substrates were formed and ion-exchange separations of solutions containing two proteins were determined by ion exchange membrane sequential separation procedures, similar to affinity membrane separation procedures. Represen...

journal_title：Journal of biotechnology

authors： Li Y,Spencer HG

更新日期：1992-11-01 00:00:00

abstract：:A serum-dependent and two serum-independent variants of the Bowes melanoma cell line, RPMI7272, were transfected with plasmids containing a geneticin-resistance (neo) gene transcribed by the HSV thymidine kinase promoter and an SV40 T antigen gene under control of the mouse metallothionein I promoter. T-antigen increa...

journal_title：Journal of biotechnology

authors： Asselbergs FA

更新日期：1992-11-01 00:00:00

abstract：:This paper discusses the concept of generic model-based software sensors with particular reference to bioprocess application. The industrial need for software sensing is considered and two industrial bioprocess systems are used in order to highlight the operability problems which warrant their development. Alternative...

journal_title：Journal of biotechnology

authors： Montague GA,Morris AJ,Tham MT

更新日期：1992-08-01 00:00:00

abstract：:There are many examples of enzymes that share substrates or cofactors in a cyclic manner. Techniques have been developed that use cyclic enzyme systems to assay quantitatively small amounts of biochemical substances (cofactor, substrate), however, only a few studies of the control of these systems have been published....

journal_title：Journal of biotechnology

authors： Okamoto M

更新日期：1992-06-01 00:00:00

abstract：:We constructed a plasmid, designated pNPP126, containing a DNA sequence encoding a fusion protein composed of Bacillus amyloliquefaciens neutral protease prepeptide (signal peptide) and human pancreatic secretory trypsin inhibitor (hPSTI), where the mature hPSTI is accurately fused to the 3'-terminal of the prepeptide...

journal_title：Journal of biotechnology

authors： Nakayama A,Kobayashi H,Ando K,Hori M,Ohnishi T,Honjo M

更新日期：1992-04-01 00:00:00

abstract：:The addition of 5-40 mM L-glutamine to batch cultures of a murine hybridoma following the cessation of rapid growth significantly stimulated monoclonal antibody (mAb) synthesis and secretion per cell. Stimulation of mAb secretion following the cessation of rapid growth was also observed in response to addition of mito...

journal_title：Journal of biotechnology

authors： Flickinger MC,Goebel NK,Bibila T,Boyce-Jacino S

更新日期：1992-02-01 00:00:00

abstract：:Vacuolar sequestration of valuable secondary metabolites remains the major limitation to the use of immobilization technology for large scale plant-cell-based bioprocesses, which otherwise may be a more efficient culture system than suspension for this biomass. In this initial study, the release of indole alkaloids pr...

journal_title：Journal of biotechnology

authors： Jardin B,Tom R,Chavarie C,Rho D,Archambault J

更新日期：1991-11-01 00:00:00

abstract：:The partitioning of proteins and other biomaterials between two aqueous phases containing polyethyleneglycol and dextran is a strong function of the molecular weight of the two polymers. Although both polymers are polydispersed (especially Dx) most theoretical treatments refer only to the average molecular weight (num...

journal_title：Journal of biotechnology

authors： Forciniti D,Hall CK,Kula MR

更新日期：1991-09-01 00:00:00

abstract：:The isolation of ars sequence from the yeast Yarrowia lipolytica has recently been reported (Fournier et al., 1991). Vectors containing ars18 have been used to increase homologous and heterologous protein production. Examples presented are the Yarrowia lipolytica alkaline extracellular protease (AEP), the porcine alph...

journal_title：Journal of biotechnology

authors： Nicaud JM,Fournier P,La Bonnardière C,Chasles M,Gaillardin C

更新日期：1991-07-01 00:00:00

abstract：:Procedures were developed for 'self-immobilisation' of Solanum aviculare cells to eliminate the need for artificial immobilisation supports. Depending on the cytokinin used in liquid medium, compact aggregates 0.4-2.0 cm in diameter were formed without dispersed cells also being present. Histochemical analysis showed ...

journal_title：Journal of biotechnology

authors： Tsoulpha P,Doran PM

更新日期：1991-06-01 00:00:00

abstract：:Sodium butyrate was used to enhance expression levels and thereby facilitate the generation of analytical quantities of nine different tissue plasminogen activator (tPA) analogues expressed under the control of the cytomegalovirus immediate early (CMV IE) promoter by the Chinese hamster ovary (CHO) mammalian expressio...

journal_title：Journal of biotechnology

authors： Palermo DP,DeGraaf ME,Marotti KR,Rehberg E,Post LE

更新日期：1991-06-01 00:00:00

abstract：:A DNA construct containing the human alpha 1-antitrypsin gene including 1.5 and 4 kb of 5' and 3' flanking sequences, was microinjected into the pronucleus of rabbit embryos. The recombinant human protein was (a) expressed in the blood circulation of F0 and F1 transgenic rabbits at an average concentration of 1 mg ml-...

journal_title：Journal of biotechnology

authors： Massoud M,Bischoff R,Dalemans W,Pointu H,Attal J,Schultz H,Clesse D,Stinnakre MG,Pavirani A,Houdebine LM

更新日期：1991-05-01 00:00:00

abstract：:The human IL-1 beta coding sequence derived from a cDNA library was inserted into two different plasmid expression vectors, pSM214 and pSM308, which promote the synthesis of recombinant products intracellularly and exocellularly, respectively. The hybrid constructs pSM261 and pSM320 were obtained. Bacillus subtilis SM...

journal_title：Journal of biotechnology

authors： Bellini AV,Galli G,Fascetti E,Frascotti G,Branduzzi P,Lucchese G,Grandi G

更新日期：1991-05-01 00:00:00

abstract：:The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

journal_title：Journal of biotechnology

authors： Weber AE,Yu P,San KY

更新日期：1991-04-01 00:00:00

abstract：:5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degree...

journal_title：Journal of biotechnology

authors： Hori N,Watanabe M,Sunagawa K,Uehara K,Mikami Y

更新日期：1991-02-01 00:00:00

abstract：:Two affinity columns comprising immobilized concanavalin A (Con A), Con A-Sepharose and Con A-XP3507, were evaluated for their purifying ability for the glycoprotein allergen Ag7 from a partially purified extract of mugwort pollen. The most pronounced difference between the two columns was the nature of their nonspeci...

journal_title：Journal of biotechnology

authors： Nilsen BM,Smestad Paulsen B,Clonis Y,Mellbye KS

更新日期：1990-11-01 00:00:00

abstract：:Peptide synthesis was carried out in a variety of organic solvents with low contents of water. The enzyme was deposited on the support material, celite, from an aqueous buffer solution. After evaporation of the water the biocatalyst was suspended in the reaction mixtures. The chymotrypsin-catalyzed reaction between Z-...

journal_title：Journal of biotechnology

authors： Clapés P,Adlercreutz P,Mattiasson B

更新日期：1990-09-01 00:00:00

abstract：:The growth inhibition of a murine hybridoma mediated by ammonium chloride was shown to vary with the pH of the culture medium. Values for the initial media concentration causing 50% growth inhibition (IC50) ranged from 4 mM to 7.6 mM as the pH was reduced from 7.8 to 6.8. A significant negative correlation was observe...

journal_title：Journal of biotechnology

authors： Doyle C,Butler M

更新日期：1990-07-01 00:00:00